Nicotinic Receptor Mediated Short Term Regulation Of In Situ Tyrosine Hydroxylase In Cultured Bovine Adrenal Chromaffin Cells (phosphorylation).

Nicotinic receptor stimulation of monolayer bovine adrenal chromaffin cell cultures results in catecholamine (CA) secretion and phosphorylation of a 56,000 dalton protein. Both events absolutely require extracellular calcium. Incubation of Western blots of electrophoretically transferred proteins from both one- and two-dimensional PAGE with specific tyrosine hydroxylase (TH) antibody resulted in t…