Single Cell Isolation from Tea Leaves and their Photosynthet…

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1979
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  • DOI 10.1626/jcs.48.343
  • OpenAlex ID W2318288016
  • Type Article
  • Language EN
  • Published 01 Jan 1979
  • Volume 48 (3)
  • Pages 343–349
  • Source Japanese Journal of Crop Science
  • Publisher Crop Science Society of Japan
  • Added 24 Jun 2016
  • Updated 31 Jul 2026
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Single Cell Isolation from Tea Leaves and their Photosynthetic Properties

Article 1979 EN Diamond

Intact mesophyll cells were isolated from tea (Camellia sinensis) leaves using an enzymatic (macerozyme) technique. About 15% of the leaf cells on a chlorophyll basis from 1 or 2 grams of leaves could be isolated in 3 hours. The optimal concentration of sorbitol was 1.0 M in the case of single cells isolated from immature leaves and 0.4 M in the case of mature leaves. Cells were slightly stable wh…

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