Isolation of Protoplasts and Chloroplasts from Flag Leaves o…

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Isolation of Protoplasts and Chloroplasts from Flag Leaves of Triticum aestivum L

Article 1979 EN Green

Intact protoplasts and chloroplasts have been isolated from mature flag leaves of wheat (Triticum aestivum L.). Both showed high rates of photosynthesis, the best of which equaled those observed in the parent tissue (greater than 150 micromoles O(2) per milligram chlorophyll per hour). The presence of ethylenediaminetetraacetate and an alkaline medium (pH 8.4) were required in the isolation and as…

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1 Richard C. Leegood University of Sheffield (GB) —
2 David A. Walker — —

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