Rapid Isolation of Intact Chloroplasts from Spinach Leaves
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- DOI 10.1007/978-1-60761-925-3_24
- PMID https://pubmed.ncbi.nlm.nih.gov/20960139
- OpenAlex ID W115689470
- Type Book-chapter
- Language EN
- Published 24 Sep 2010
- Volume 684
- Pages 321–325
- Source Methods in molecular biology
- Publisher Springer Science+Business Media
- FWCI 1.17
- Countries 1
- Institutions 1
- Added 24 Jun 2016
- Updated 31 Oct 2026
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Rapid Isolation of Intact Chloroplasts from Spinach Leaves
Book-chapter 2010 EN
In this chapter, a rapid method to isolate intact chloroplasts from spinach leaves is described. Intact chloroplasts are isolated using two short centrifugation steps and avoiding the use of percoll gradient. Intactness of chloroplast is evaluated by the inability of potassium ferricyanide to enter inside the chloroplasts and to act as an electron acceptor for photosystem II.
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2 authors| # | Author | Institutions | ORCID |
|---|---|---|---|
| 1 | David L. Joly | Université du Québec à Trois-R… (CA) | |
| 2 | Robert Carpentier | Université du Québec à Trois-R… (CA) | — |
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